Amino acid composition analysis is a classical protein analysis method. It can be used to determine the composition and content of amino acid in protein/peptide, and non-typical amino acid in protein/peptide drugs. Analysis of amino acids is a necessary step before the complete sequencing of biopharmaceuticals, and provides valuable evidence for the analysis of the sequence of biopharmaceuticals.
The service mainly consists of two steps: (1) Hydrolysis of proteins and peptide samples into free amino acids; (2) The derivation and detection of free amino acids.
APTBIO uses phenyl isothiocyanate (PITC) for amino acid derivation, followed by a high-efficiency liquid chromatography for determining the composition and content of amino acid derivatives, so as to speculate the components of the amino acids in the protein/peptide samples.