DIGE

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) is a gel-based approach for comparative proteomics using fluorescent tags. Distinct fluorescent tags e.g. Cy 3, 5 and 2 are used to label samples and a universal internal standard prior to 1st/2nd dimension electrophoresis. An automated software program is used to detect, quantify and annotate differentially expressed proteins. 2D-DIGE offers all the advantages of 2D-PAGE and overcomes the inherent disadvantage of variation and reproducibility problem in a 2D-PAGE.


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Bioinformatics analysis:

1) Protein GO analysis

2) Protein KEGG analysis

3) Differentially expressed proteins GO enrichment analysis

4) Differentially expressed proteins KEGG enrichment analysis

5) Differentially expressed proteins cluster analysis

6) Differentially expressed proteins PPI analysis


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